Re: AAA hydrolysis

Len Packman (lcp2@mole.bio.cam.ac.uk)
Fri, 22 Jan 1999 09:16:49 +0000

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Harold G. Hills: "DNA seq 96 lane upgrade"
Previous message: Loc-Ha Le: "line in gel file"
In reply to: tandersen@ccgateway.amc.edu: "Re: AAA hydrolysis"

I would advise caution over interpreting results from samples with
carbohydrate tar. We have certainly seen both reduced recovery and
selective amino acid loss from such samples. If the sample cannot be
cleaned up, then I would strongly advise running a standard protein,
hydrolysed from the same buffer, and assess recovery of the amino acids vs
expected values.

Len

*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html

Next message: Harold G. Hills: "DNA seq 96 lane upgrade"
Previous message: Loc-Ha Le: "line in gel file"
In reply to: tandersen@ccgateway.amc.edu: "Re: AAA hydrolysis"