we have the upgrade since some time, and in principle it did work in our
hands. We load the wells one-by-one, and under these conditions the versi=
on
3.x tracker works OK for us. However, we detected a significant loss of
read length on our 96-gels compared to other gels. I have no exact idea w=
hy
there is this reduction, but I asume that the lanes are to narrow to buil=
d
up a real "band" in the gel, and the result is "blobs" of signal running
accross the detection zone. In addition, even the best tracking has troub=
le
in finding the centre of the lane in terms of data points on the gel imag=
e,
and also this may contribute the problem.
In practice, we now use 64-lane gels with the step-plates. The comb used =
is
the one from the 373 PE/Abi equipment. The result is 0.4 mm "thick" wells
which are realy easy to load. We reach good read length, and the gel
performance is OK.
One other point: During trying the PE/Abi 1000+ protocols, we very often
have spacing values which are too high for the automatic analysis softwar=
e.
This results in a "red" spacing value "9.00". It's due to the fact that t=
he
(sorry - stupid!!) software can not handle spacing values higher than 16
(why does PE/Abi recommend protocols which result in data which their own
software can not work with??). Has anybody a solution for this problem?
(beside setting the spacing value by hand to 17.00)
Best regards, Bernd
>Hi all,
>
>I am interested in communicating with people who are routinely using or
>attempting to use the Perkin-Elmer 96 lane upgrade. We have been workin=
g
>with the upgrade since October and are still having problems; namely, ho=
w
>to flush and load the wells. We have tried several methods but even the
>most promising one appears to cause leaking of the wells which in turn
>causes the tracking to be a total nightmare!!
>
>I am also interested in speaking with people who are ROUTINELY using the=
48
>and/or 64 lane upgrades as well.
>
>What are your opinions and experiences regarding these extra lane upgrad=
es
>(48, 64, and 96)?
>
>Sincerely,
>
>
>Wendy Fuller
>Research Specialist, DNA Services
>University of Illinois
>W.M. Keck Center for Comparative & Functional Genomics
>330 Edward R. Madigan Lab, MC 051
>1201 W. Gregory St.
>Urbana, IL 61801
>(217) 333-9520
>fax: (217) 265-5066
>wfuller@ux1.cso.uiuc.edu
_____________________________________________________________________
PD Dr. Bernd Weisshaar
MPI fuer Zuechtungsforschung
Carl-von-Linn=E9-Weg 10
50829 Koeln
Germany
Tel. office: +49-221-5062 590 Tel. lab: +49-221-5062 311
Fax: +49-221-5062 313
e-mail: weisshaa@mpiz-koeln.mpg.de
WWW-URL: http://www.mpiz-koeln.mpg.de/~weisshaa/BW-info.html