I can't answer directly but may be able to give some insight. DTT
will reduce oxidized BME because DTT's reduction potential is more
negative. Oxidized BME is thought to help catalyze disulfide bond
formation in proteins, and oxidized DTT has the reputation of being much
less reactive in this regard. Scopes' book on protein purification
discusses this as well as anyone's. These two properties make DTT superior
when cost is not a factor. This may or may not answer the question of why
one might have both present at the same time.
Hope this helps.
Chris
Hi, all,
A chemistry question
I have someone who runs their protein preps in low concentration of BME
and DTT (with basically a PBS recipe at pH 7)--is their some
advantage/disadvantage to doing this. Does BME or DTT work on the
other?
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427