I just put a sample (short tryptic peptide on a Biobrene treated filter) on
the protein sequencer and saw nothing for the 1st 4 residues. Then suddenly
in the 5th residue cycles numerous AA's appeared and I am now getting
sequence. The 5th residue looked very much like a typical 1st residue cycle
with high levels of Glu and gly. Has anyone ever seen this before and what
causes it?
thanks a lot
Amanda Hall
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Amanda Hall (BSc. Hons)
Professional Officer
Newcastle Protein
University of Newcastle
ph 02 4921 7299
fax 02 4921 6903
email: mbah@medicine.newcastle.edu.au
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