Re: Varian vs Waters for prep LC of peptides

David A. Schooley (schooley@unr.edu)
Wed, 27 Jan 1999 08:14:53 -0800 (PST)

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James-
Don't rule out a ThermoSeparations Products (formerly Spectra
Physics) P4000 with prep heads. We have one with their VW detector with 1
mm prep cell. Certainly for prep work you do NOT want more than a 3 mm
cell, and a 1 mm is better. The prep heads are PEEK, so the whole system
is "biocompatible". I believe it will deliver up to 30 ml/min, but we use
20 max on 1" OD columns. The pressure limitation with prep heads is 2,000
psi, but we are generally using 10 micrometer packings in prep
chromatography so this is no problem. The unit has performed reliably for
us and is easy to use.

David

David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (775) 784-4136; fax (775) 784-1419

Next message: Holyst, Trudy: "Eqpsale"
Previous message: Thomas J Miller: "Re: Prot Seq: leaky guard column"
Maybe in reply to: James Ferguson: "Varian vs Waters for prep LC of peptides"