Digest
Ulf Hellman (ulf.hellman@licr.uu.se)
Wed, 03 Feb 1999 09:41:36 +0200
Dear all,
A colleague of mine needs to digest (trypsin) a radioactive sample sitting
in a nitrocellulose membrane. Unfortunately the membrane had dried and that
may be why his first attempt, using the Aebersold protocol, to isolate
peptides failed? Can anybody suggest a way to better yield?
Thanks for help /Ulf
******************************************************
Ulf Hellman, Ph. D.
Adj. Prof.
Ludwig Institute for Cancer Research :
Box 595
S-751 24 Uppsala
Sweden
Phone +46 18 160423
Fax +46 18 160420
E-mail ulf.hellman@licr.uu.se
E-mail (alt.) cykeldoktorn@hotmail.com
Lab's home page: http://www.licr.uu.se