Digest on nitrocellulose
bch143@abdn.ac.uk
Wed, 3 Feb 1999 11:37:55 +0000 (GMT)
Ulf,
I have had considerable success using the method of Lui, Tempst and
Erdjument-Bromage. Anal Biochem. 241, 156-166 (1966). You have to get a
detergent (Zwittergent 3-16) from Aldrich. It doesn't seem to matter if the NC
has dried or not.
Regards,
Bryan.
--
Bryan Dunbar
Protein Facility
Dept. of Molecular & Cell Biology
University of Aberdeen
Polwarth Building
Foresterhill
Aberdeen
AB25 2ZD
Tel:- +44 (0)1224 273103
Fax:- +44 (0)1224 273104
e-mail:- b.dunbar@abdn.ac.uk