RE: Add an acetyl group to a purified peptide
James Ferguson (JamesF@minimed.com)
Wed, 3 Feb 1999 10:21:01 -0800
Will this not also acetylate the side chain of lysine, and perhaps affect
other residues such as arginine as well?
>
> Bob,
>
> our standard protocol is copied below.
>
> Good luck.....Ken
>
> 1. Dissolve sample in 100 microlitres water
> 2. Vortex and add 10 microlitres triethylamine
> 3. Vortex and add 5 microlitres acetic anhydride
> 4. Vortex
> 5. Dry in Speedivac
>
>
> 6. Dissolve sample in 100 microlitres water
> 7. Vortex and add 10 microlitres piperidine
> 8. Incubate at room temperature for 60 minutes
> 9. Dry in Speedivac
>
>
> >Hi everyone,
> >
> >Please give me a protocol to add an acetyl group at the N-terminus of a
> >peptide which has already been cleavaged and purified by HPLC. Thanks.
> >
> >Best Regards,
> >
> >Bob Lee
>
>
>