<smaller>
</smaller></fontfamily><smaller><fontfamily><param>New_York</param>Ulf,</fontfamily><fontfamily><param>Times</param>
</fontfamily><fontfamily><param>New_York</param>This protocol works
well</fontfamily><fontfamily><param>Times</param>.
</fontfamily><fontfamily><param>New_York</param>Analytical
Biochemistry, 1992, Vol 201, pp. 255-264 Fernandez et. al. "Internal
Protein Sequence Analysis: Enzymatic Digestion for Less Than 10 ug of
Protein Bound to Polyvinylidene Difluoride or Nitrocellulose
Membranes."</fontfamily><fontfamily><param>Times</param>
</fontfamily><fontfamily><param>New_York</param>Dick
Cook</fontfamily><fontfamily><param>Times</param>
</fontfamily></smaller>
Dear all,
A colleague of mine needs to digest (trypsin) a radioactive sample
sitting
in a nitrocellulose membrane. Unfortunately the membrane had dried and
that
may be why his first attempt, using the Aebersold protocol, to isolate
peptides failed? Can anybody suggest a way to better yield?
Thanks for help /Ulf
******************************************************
Ulf Hellman, Ph. D.
Adj. Prof.
Ludwig Institute for Cancer Research :
Box 595
S-751 24 Uppsala
Sweden
Phone +46 18 160423
Fax +46 18 160420
E-mail ulf.hellman@licr.uu.se
E-mail (alt.) cykeldoktorn@hotmail.com
Lab's home page: http://www.licr.uu.se