Microphysiometer and peptide purification questions

Hong Li (HLi@synapticcorp.com)
Wed, 3 Feb 1999 10:03:38 -0500

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Dear all:

Regarding microphysiometer/cytosensor:=20
1. Does anyone have experience with using microphysiometer for high =
throuput screening?=20
2. Has anyone tried to minitrarize the plumbing of microphysiometer to =
improve assay sensitivity, thus use less ligand for receptor assay?

Regarding peptide purification:
1. Are there any proven means to prevent peptides from sticking to the =
tubes besides using siliconized tubes and BSA etc?
2. Can someone comment on their experience using pGlu aminopeptidase or =
acylamino-acid releasing enzyme for liberating blocked N-terminal AA?

Thanks in advance for your input,=20

Hong Li
Synaptic Pharmaceutical Corp
Paramus, NJ=20

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Dear all:
 
Regarding = microphysiometer/cytosensor:=20
1. Does anyone have experience with = using=20 microphysiometer for high throuput screening?
2. Has anyone tried to minitrarize = the plumbing=20 of microphysiometer to improve assay sensitivity, thus use less ligand = for=20 receptor assay?
 
Regarding peptide = purification:
1. Are there any proven means to = prevent=20 peptides from sticking to the tubes besides using siliconized tubes and = BSA=20 etc?
2. Can someone comment on their = experience using=20 pGlu aminopeptidase or acylamino-acid releasing enzyme for liberating = blocked=20 N-terminal AA?
 
 
Thanks in advance for your=20 input, 
 
Hong Li
Synaptic Pharmaceutical = Corp
Paramus, NJ 
 
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