Richard Laursen
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>Hi,
>
>My name is Eric Tam and I'm a biochemistry PhD student here at the
>University of British Columbia in Vancouver, Canada. I was recommended this
>address by the Nucleic Acids and Protein Services (NAPS) of UBC. I have a
>question regarding biotin modification and trypsin digestion.
>
>If a primary amine is modified by a biotinylation reagent (say from Pierce),
>is trypsin able to cleave at this modified site? The reason I'm asking this
>is that I'm proposing to biotinylate a protein to identify surface exposed
>Lys residues. I then want to be able to digest the protein and sequence
>only the biotinylated peptides.
>
>Your response is greatly appreciated,
>
>Eric Tam
Richard A. Laursen
Department of Chemistry
Boston University
590 Commonwealth Ave.
Boston, MA 02215
Tel (617) 353-2491; FAX (617) 353-6466
email: <laursen@bu.edu>