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Subject: desalting
Author: novick@immunex.com at internet
Date: 2/4/99 7:48 PM
Hi All,
I am reducing and alkylating proteins in a guanidine solution and then
buffer exchanging using the BioRad P6 centrifuge columns. Our group is
looking for a faster, easier way to clean the sample (we can have many
samples to desalt at a time). RP desalting doesn't work (protein
precipitates and the protocol is WAY too long). I'm interested in some
kind of desalting pipet that will make this a none-issue for the QC
and Formulations folk. Are there any suggestions? The final solution
should be in a neutral, enzyme-friendly, somewhat buffered solution.
Thanks in advance for your help,
Shawn Novick