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Emne: Re: desalting
Forfatter: kimber@nnbt.com p=E5 INTRANOTES
Dato: 05-02-99 18:19
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I haven't tried them. But I just got an ad from Millipore abou=
t a=20
ZipTip Pipette Tip. Here's the ad: Concentrate,desalt and fract=
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from 1-100ul in less than one minute. Pipette tip with 0.6ul o=
f C18=20
resin.
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Any one tried these yet?
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-Kim Brown
Novo Nordisk Biotech, Inc.
kimber@nnbt.com
www.nnbt.com
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______________________________ Reply Separator=20
_________________________________ Subject: desalting
Author: novick@immunex.com at internet=20
Date: 2/4/99 7:48 PM
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Hi All,
I am reducing and alkylating proteins in a guanidine solution a=
nd then=20
buffer exchanging using the BioRad P6 centrifuge columns. Our g=
roup is=20
looking for a faster, easier way to clean the sample (we can ha=
ve many=20
samples to desalt at a time). RP desalting doesn't work (protei=
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precipitates and the protocol is WAY too long). I'm interested =
in some=20
kind of desalting pipet that will make this a none-issue for th=
e QC=20
and Formulations folk. Are there any suggestions? The final sol=
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should be in a neutral, enzyme-friendly, somewhat buffered solu=
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Thanks in advance for your help,
Shawn Novick
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