Re: Short peptide

J.Hardy (j.hardy@ukc.ac.uk)
Tue, 9 Feb 1999 17:45:50 -0500 (EST)

We have made quite a number of short (around 6 aa)
peptides with Fmoc chemistry. After cleavage as usual you
can dilute the cleavage mixture with water (5x vol of
cleavage mix) and extract with a similar volume of di-ethyl
ether to remove scavengers. Remove ether layer, repeat
several times, and then freeze dry the aqueous layer.

Judy Hardy
Department of Biosciences
The University of Kent
Canterbury
Kent CT2 7NJ
U.K.
Tel: +44 (0)1227 764000
Fax: +44 (0)1227 763912
email: J.Hardy@ukc.ac.uk
On Tue, 9 Feb 1999 06:16:38 -0600 (CST) Bao-Shiang Lee
<boblee@uic.edu> wrote:

> Hi everyone,
>
> I am trying to synthesize two short peptides (1. c(amc)vgc(amc) and 2.
> avga). After cleavage, they can not be precipitated by ether. Mass spectra
> show they are there. Any suggestions would be appriciated.
>
> Best regards,
>
>
> Bob Lee

----------------------
Judy Hardy
J.Hardy@ukc.ac.uk