I've used the PEG resins from Millipore and the Tentagel resins from
Novabiochem. This was using them for Fmoc chemistry on manual and
semi-automatic continuous-flow synthesizers. Both resins work nicely -
they give better reaction kinetics (qualitatively, no kinetic experiments
performed), with fewer steps requiring repeat acylations or blocking (this
is with peptides varying in length from 12 to 46 amino acids). Products
also seem cleaner. This is all in comparison to resins with the same sort
of linkers but without the
PEG spacer arms.
Qualitatively, they also seem to handle better in the flow synthesizers,
with less episodes of resin compaction.
They are, however, much harder to wash post synthesis, in the sense that
they hang on to ether tenaciously and require very thorough vaccum drying!
Hope this helps, I'll be interested to see if other people have found these
resins useful too.
Roger
At 08:59 AM 09-02-99 -0500, you wrote:
> ABRF members,
>
> I would love to hear your remarks/success or failure stories with
> the PEG and/or Tentagel resins. Are they indeed the same resin just
> supplied from different vendors?
>
> If you have used either or both I would appreciate your passing
> along comments especially as they pertain to usage on the Rainin
> Symphony instrument.
>
> Thanks,
>
> Millie McAdams/ HHMI Biopolymer Facility
> 919-684-2652 FAX: 919-684-5458
> mcada002@mc.duke.edu
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au