Try Peptide Companion from Coshisoft/Peptisearch - it's got some useful
features for looking at the properties of peptides, including just what you
ask about. It gives you the choice of several different scales to use for
prediction at both pH 2 and pH 7. I believe it's only about US$70-80, but
search Coshisoft on the web and check out their current price list.
Roger
At 03:37 PM 09-02-99 -0800, you wrote:
>Hi, all,
>
>Does anyone know of a program that we could use to predict the elution
>order of peptides from RP-HPLC (pH 2.0 and/or pH 7.0) --we would like to
>be able to predict the approximate change in retention (well, position
>in the chromatogram) time--for peptides fragments with different amino
>acid substitutions.
>
>Thanks for any suggestions,
>Deb McMillen
>Institute of Molecular Biology
>University of Oregon
>Eugene OR 97403
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au