Re: HPLC

Ulf Hellman (ulf.hellman@licr.uu.se)
Wed, 10 Feb 1999 09:41:42 +0200

Hi Deb,
We do use GPMAW for this and other purposes. You can for example make a dry
digest of a protein and get a table of fragments with a lot of details for
them. One of the columns indicate a relative retention time of the
peptides, calculated as in:
Y. Sakamoto, N. Kawakami & T. Sasagawa, J. Chrom. 442, 69-79 (1988).
By a press of a button, GPMAW can also display a chromatogram, in which one
can type in a peptide sequence and see where it would elute. (For example,
you can make a mutation and see in which direction the "new" peptide moves
in the chromatogram.) One can also use another author's retention time
predictions: (Meek, PNAS 77, 1632-1636, 1980). Strange enough, they differ
quite much... In the Meek paper, you can predict retention at low and high pH.
You can find GPMAW in the web.
I do not have shares in GPMAW, but I like the program!
/Ulf
******************************************************
Ulf Hellman, Ph. D.
Adj. Prof.
Ludwig Institute for Cancer Research :
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