Re: TMA leaks

Ricardo Bastos Cunha (rbcunha@guarany.cpd.unb.br)
Wed, 10 Feb 1999 17:54:14 -0200

Sandy, N-methylpiperidine can be used instead of TMA for PITC coupling, and
has many advantages others than the aroma. It's much less volatile than TMA
and more stable, resulting in a longer life time on the flask. The pKa of
N-methylpiperidine is very close to that of TMA and it has only one
degradation product: piperidine. The most important thing is that
N-methylpiperidine provides a better speed and more efficiency of coupling
than TMA. Convinced?

Sandy Kielland wrote:
> Greetings ABRF'ers:
> Has anyone come up with a way to seal the joint around the tubing going
> into the acid trap on ABI protein sequencers? My lab constantly smells of
> TMA and this doesn't make me very popular with my coworkers.
> Is there any advantage to switching to a different base instead of TMA
> (other than the aroma)?
> Regards,
> Sandy Kielland
> Protein Chemistry Centre
> Department of Biochemistry and Microbiology
> University of Victoria
> CANADA

-- 
Best regards,


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
Ricardo Bastos Cunha      Brasilia - DF - Brasil
                          mailto:rbcunha@unb.br
                          http://www.geocities.com/Athens/Acropolis/5221
Instituto de Quimica - UnB
                          http://www.unb.br/ie/qui/
Centro Brasileiro de Servicos e Pesquisas em Proteinas - UnB
                          http://www.unb.br/cbsp/CBSP.html
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