The HPLC prediction algorithms are as useful or useless as you let them be
- I've only ever used them as a guide when my own experience needed a
little back-up! It's wise to always use several different predictors so
that you get a feel for how the peaks may move around - and always be
prepared for the unexpected!
I'm sure this doesn't help much - experience is usually a much better guide.
Roger
At 11:49 AM 10-02-99 -0500, you wrote:
>
>To all,
>
>Regarding prediction of peptide elution, and in light of Ulf's comment that
>the
>prediction varies with the program used, I was wondering to what extent
people
>using them have found them helpful?
>
>Linda
>
>=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
>Linda Siconolfi-Baez
>Core Protein Sequencing Center
>SUNY Health Science Cntr.
>450 Clarkson Ave.
>Box 8
>Brooklyn, N.Y. 11203
>718-270-1267 (phone)
>718-270-3316 (fax)
>lsiconolfi-baez@netmail.hscbklyn.edu
>http://www.hscbklyn.edu/SUNY/Biochem/Bioparts/ProtSeq.html
>=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=-=
>
Roger Murphy, Ph.D.
Biological Production Facility
Ludwig Institute for Cancer Research
Austin & Repatriation Medical Centre
Studley Road,
Heidelberg, Vic. 3084
Australia.
Tel 61-3-94965463
Fax 61-3-94965436
Email Roger.Murphy@Ludwig.edu.au