RE: DNASeq:Upgrade to 877 Turbo Catalyst

Margaret Robertson (margaret.robertson@hci.utah.edu)
Wed, 10 Feb 1999 18:23:11 -0700

Ernesto, I would not waste $15,000 on a Turbo upgrade. Our Catalyst 800 was
converted to Turbo and is STILL a big paperweight! It is much faster to
assemble dye terminator reactions manually using multichannel pipettes and
PCR machines. We use MJ cyclers and a combination of multichannel and combi
tip pipettes. We can process 4x96 samples easily in a few hours where it
takes the catalyst much longer and you can only process 192 dye terminator
samples at one time. We used our Catalyst more in the old days to process
dye primer reactions, but have given up on these to go entirely over to Big
Dye terminators now.

Margaret Robertson
Director, DNA Sequencing Facility
University of Utah

> ----------
> From: Ernesto C. Almira, Ph.D.
> Reply To: ernie@bitech.ufl.edu
> Sent: Wednesday, February 10, 1999 6:26 PM
> To: Recipients of ABRF List
> Subject: DNASeq:Upgrade to 877 Turbo Catalyst
>
> Hi All,
>
> I am thinking of upgrading my old 800 catalyst (which right now is just
> a big paper weight) to an 877 Turbo Catalyst. I would like to know your
> experience regarding this model or $15K upgrade. We only do dye
> terminator reactions using both standard M13 primers and custom primers,
> and are using both Taq FS and BigDye kits .
>
> I appreciate very much any suggestions/help.
>
> Sincerely,
>
> Ernesto
> ***************************************
> Ernesto C. Almira, Ph.D.
> Director
> DNA Sequencing Core Facility
> The Biotechnology Program
> University of Florida
> 2301 Fifield Hall
> Gainesville, FL 32611-0695
> Voice: (352) 392-6260
> Fax: (352) 392-4072
> e-mail: ernie@biotech.ufl.edu
> web:
> http://www.biotech.ufl.edu/~dseqcl
> ***************************************
>