See Thannhauser et. al. J. of Protein Chemistry 17, 37-43, 1998. They use
AAA to do the analysis. If you only want to know there is disulfide
bridges or not (you do not have enough protein), One trick that we use is
that digest you protein with CNBr and run SDS-PAGE one with the reducing
agent (DTT or BME) and one without reducing agent. Compare the fragments.
This work for us on HEW lysozyme. Use silver stain ( be careful some of
the protein won't stain well) to improve the sensitivity. Good Lock.
Best regards,
Bob Lee
Director of the Protein Research Lab. at UIC
On Wed, 10 Feb 1999, Maria Lorenzi wrote:
> Hi, I'm Maria Lorenzi.
> I would like to know if there is a procedure for the determination of
> disulfide bridges of a protein, having little amount of it.=20
> I know the method whith Ellman reagent but it is used when you have eno=
ugh
> protein.
> Please, let me know
> Maria=20
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> "Donde hay Fe hay Amor, Donde hay Amor hay Paz
> Donde hay Paz esta Dios y donde esta Dios
> no falta nada"
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> ***********************************************************************=
***
> Dott.ssa Maria Lorenzi
> Dottoranda in Biochimica e Biofisica
> Istituto di Biochimica
> Facolt=E0 di Medicina e Chirurgia
> Facolt=E0 di Agraria
> Universit=E0 degli Studi di Ancona
> Via Ranieri 60131 Ancona, Italia
> Tel.:+39 71 220 4677
> +39 71 220 4681
> Fax: +39 71 280 2117
> e-mail: mlorenzi@ascu.unian.=
it
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