Fwd: DNASeq: chemistry option

George S. Grills (grills@aecom.yu.edu)
Thu, 11 Feb 1999 19:03:44 -0500

This is exactly the type of question asked by the ABRF DNA Sequencing Research
Committee 1999 Survey. The first part of the survey involves a "Never Ending
Story" study with a standard pGEM template. Information on how to submit data
for this part of the study is posted at
http://mbcf.dfci.harvard.edu/dsrc.html.
The second part of the study involves the results of sequencing challenging
templates with different conditions, including both Amersham and ABI dye
chemistries. Instructions on how to request samples and submit data can be
found at the Difficult Template Study web site at
http://brcweb.bio.cornell.edu/dsrc99.

- The ABRF DNA Sequencing Research Committee

>Date: Thu, 11 Feb 1999 10:36:18 -0800
>Old-To: abrf@aecom.yu.edu
>From: Martin Pentony <mpento@cmgm.stanford.edu>
>Subject: DNASeq: chemistry option
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Sender: Association of Biomolecular Resource Facilities
><abrf-request@aecom.yu.edu>
>
>I'm also interested in comments on the DYEnamic ET terminator. At present
>we are using BigDyes. I had some problems with secondary structures. I
>tried raising the extension temperature to 70 but the reactions failed.
>This had worked before with Amersham Rhodamine kits. Have the ABI kits a
>lower temperature tolerance? My impression is that the amersham ET
>terminators work as well as the BigDyes and are better for difficult
>templates like secondary structure and poly A or poly T. Comments from the
>experienced would be most welcome. Thanks
>Martin Pentony
>DNA Sequencing section
>P.A.N. Facility
>Stanford University
>http://cmgm.Stanford.EDU/pan/
>
>