Trypsin is not a good choice. This produces too many short frgaments and
you can not use SDS-PAGE at all (you can try limited digestion). You can
figure out which cysteines form disulfide bridges easily because you know
the sequence of your protein. You have many choices to do the digestion o=
n
orther residues. Please post a message one more time with your sequence.
There sre many experts out there to help you. Hope this help.
Best regards,
Bob Lee
Director of PRL at UIC
On Fri, 12 Feb 1999, Maria Lorenzi wrote:
> Hi, I'm Maria Lorenzi.
> Thanks for your advises.=20
> I can do the experiment but I cannot digest my protein with CNBr becaus=
e
> methyonines are not present in my protein; I can use trypsin for this
> purpose and probably it will be good.
> I know the sequence of my protein and that there are six cysteines on i=
t but
> I would like to know the number of the disulphide bridges; do you think=
that
> with your method I can know it?
> Anyway, thanks a lot=20
> Maria
>=20
>=20
>=20
>=20
>=20
>=20
>=20
>=20
>=20
> "Donde hay Fe hay Amor, Donde hay Amor hay Paz
> Donde hay Paz esta Dios y donde esta Dios
> no falta nada"
>=20
> ***********************************************************************=
***
> Dott.ssa Maria Lorenzi
> Dottoranda in Biochimica e Biofisica
> Istituto di Biochimica
> Facolt=E0 di Medicina e Chirurgia
> Facolt=E0 di Agraria
> Universit=E0 degli Studi di Ancona
> Via Ranieri 60131 Ancona, Italia
> Tel.:+39 71 220 4677
> +39 71 220 4681
> Fax: +39 71 280 2117
> e-mail: mlorenzi@ascu.unian.=
it
>=20