DNASEQ: 96-well DNA prep protocols

Robert Lyons (boblyons@umich.edu)
Fri, 12 Feb 1999 13:32:39 -0500

I have a client who wants to submit 500 clones for sequencing,
and would appreciate not having to (a) run manual minipreps
on all of them, or (b) buy a robot or other expensive apparatus
for this one chore. The clones are currently in five 96-well
plates, presumably as bacterial stocks.

Does anyone have suggestions on how they should proceed? Any
experience with 96-well miniprep methods that would give good,
sequencable template and yet not cost too much? Source for
protocols?

Thanks for any suggestions.

Bob Lyons

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Robert Lyons, Ph.D.
Director, DNA Sequencing Core
University of Michigan
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