I was sequencing a strange plant protein the other day when my nice
easy-to-read sequence suddenly produced an unrecognizeable amino acid for 6
cycles before returning to standard amino acids. Some 8 residues later another
string of 5 more of this unknown appeared. It elutes as three peaks on a
Procise with buffer A3/B2/premix. The biggest elutes after ALA and the other
two, which are half its size, elute between GLU and HIS. Can anyone offer some
possible clues as to the identification of this.
Many thanks in advance
Mike
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Dr Mike Naldrett
Protein Sequencing & Peptide Synthesis Facility
John Innes Centre(NFL)
Norwich Research Park
Colney
Norwich
NR4 7UH
tel 01603-456900 x 2773/2724
fax 01603-454970
e-mail: mike.naldrett@bbsrc.ac.uk