Re: DNASEQ: 96-well DNA prep protocols

Sheryl Christofferson (sherylc@omrf.ouhsc.edu)
Mon, 15 Feb 1999 13:50:44 -0500

Hi Bob-
One of my clients uses the Qiagen 96 plasmid prep kit. The
cultures are grown in a 96 tube plate, and purified in the same
plate. We have had about 99% success rate. Unfortunately, this
client is not in today, but I can get specifics soon.

Hope this helps.

Sheryl Christofferson
Oklahoma Medical Research Foundation
Oklahoma City, OK
sherylc@omrf.ouhsc.edu

Robert Lyons wrote:

> I have a client who wants to submit 500 clones for sequencing,
> and would appreciate not having to (a) run manual minipreps
> on all of them, or (b) buy a robot or other expensive apparatus
> for this one chore. The clones are currently in five 96-well
> plates, presumably as bacterial stocks.
>
> Does anyone have suggestions on how they should proceed? Any
> experience with 96-well miniprep methods that would give good,
> sequencable template and yet not cost too much? Source for
> protocols?
>
> Thanks for any suggestions.
>
> Bob Lyons
>
> -----------------------------
> Robert Lyons, Ph.D.
> Director, DNA Sequencing Core
> University of Michigan
> -----------------------------