You may want to look up the following current reference where they used
96-Well spin plates for the isolation of DNA from dried blood spots:
Anal Biochem 267 (2) 415-418 (1999) Tsing Su & Anne Comeau "Cellulose as a
Matrix for Nucleic Acid Purification".
Regards,
Amos Heckendorf
The Nest Group
>-----Original Message-----
>From: Robert Lyons <boblyons@umich.edu>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Date: Friday, February 12, 1999 4:00 PM
>Subject: DNASEQ: 96-well DNA prep protocols
>>I have a client who wants to submit 500 clones for sequencing,
>>and would appreciate not having to (a) run manual minipreps
>>on all of them, or (b) buy a robot or other expensive apparatus
>>for this one chore. The clones are currently in five 96-well
>>plates, presumably as bacterial stocks.
>>
>>Does anyone have suggestions on how they should proceed? Any
>>experience with 96-well miniprep methods that would give good,
>>sequencable template and yet not cost too much? Source for
>>protocols?
>>Robert Lyons, Ph.D.
>>Director, DNA Sequencing Core
>>University of Michigan
Amos Heckendorf (nestgrp@world.std.com)
The Nest Group, Inc., Value Added Resellers of HPLC Columns (Vydac(tm),
PolyLC(tm), BioChrom Hydrocell(tm), Jordi-Gel(tm), Macherey-Nagel
Nucleosil(tm), Higgins Analytical HAISil(tm) & TARGA(tm), and Bischoff
Prontosil(tm)) genomic Solutions(tm) PAGE gels; Amika COZAP Destaining
pads, Dispo-BioDialyser(tm) and ProTip(tm) MS Micro Sample SPE; and KronLab
BioCart(tm) glass adjustable columns. Tel: 800-347-6378 or 508-481-6223;
FAX 508-485-5736;
45 Valley Rd, Southboro, MA 01772-1306
Applications and Protocols at:
HTTP://world.std.com/~nestgrp/protocols/protocol.html