Other websites of interest would be at centers doing large scale or genome
sequencing projects. You might also check Millipore's 96-well Multiscreen
protocol for DNA isolation.
Good Hunting!
Al Cairo
******************
Alfred M. Cairo
BACPAC Resources
DNA Sequencing Lab
Dept. of Cancer Genetics
Roswell Park Cancer Institute
Buffalo NY 14263
email: cairo@dejong.med.buffalo.edu
web site: http://bacpac.med.buffalo.edu
phone: 716-845-4543
-----Original Message-----
From: Robert Lyons <boblyons@umich.edu>
To: Recipients of ABRF List <abrf@aecom.yu.edu>
Date: Friday, February 12, 1999 4:00 PM
Subject: DNASEQ: 96-well DNA prep protocols
>
>I have a client who wants to submit 500 clones for sequencing,
>and would appreciate not having to (a) run manual minipreps
>on all of them, or (b) buy a robot or other expensive apparatus
>for this one chore. The clones are currently in five 96-well
>plates, presumably as bacterial stocks.
>
>Does anyone have suggestions on how they should proceed? Any
>experience with 96-well miniprep methods that would give good,
>sequencable template and yet not cost too much? Source for
>protocols?
>
>Thanks for any suggestions.
>
>Bob Lyons
>
>-----------------------------
>Robert Lyons, Ph.D.
>Director, DNA Sequencing Core
>University of Michigan
>-----------------------------
>
>