Re: 2 d gel service

Wayne Patton (WaynePatton@probes.com)
Thu, 18 Feb 1999 15:13:11 -0800

Dear David,
A number of years ago I developed such a system with other scientists at
Millipore Corporation.

Patton, W., M. Pluskal, W. Skea, J. Buecker, M. Lopez, R. Zimmerman, L.
Belanger, and P. Hatch (1990). "Development of a dedicated two-dimensional gel
electrophoresis system that provides optimal reproducibility and polypeptide
resolution." BioTechniques 8: 518-527.

The system has been updated and integrated into a modern proteomics platform by
Genomic Solutions, 22 Alpha Road, Chelmsford, MA. The Proteomics VP is Dr. Mary
Lopez (978) 244-0033, Extension 12. Dr. Lopez runs a gel service laboratory and
can provide you with alot of personal experiences on using the equipment in such
a capacity. I ran a facility using the equipment for a few years while at
Memorial Sloan-Kettering Cancer Center in New York and later at Boston
University. I routinely use it now in the development of new fluorescent
detection reagents for 2-D gel electrophoresis (the new SYPRO Ruby dyes). If
the big, 22 X 22 cm gels are not what you had in mind, the company has developed
a mini-2D system as well.

Wayne F. Patton
Group Leader
Bioanalytical Assay Development
Molecular Probes, Inc.
4849 Pitchford Avenue
Eugene, OR 97402

"Chin, David T." wrote:

> I'm looking for an easy 2 d gel system that would be used in a service
> operation. I'm looking for ease of use and low cost of operation (including
> labor). Any suggestion on the different makes?
>
> David.
>
> David T. Chin
> Director, Protein Core Facility
> Univ. of Missouri - Columbia
> Columbia, MO 65211
>
> SHIPPING AND ACTUAL LOCATION:
> 2-17 Agriculture Building (office)
> 2-31 Agriculture Building (Lab)
>
> e-Mail: chind@missouri.edu
> web: http://www.missouri.edu/~pc
> [mailto:chind@missouri.edu]
> Phone: 573-882-2027
> Fax: 573-882-7105