Antifreeze protein type III

david andreu (andreu@admin.qo.ub.es)
Wed, 24 Feb 1999 16:39:15 +0100

Hi all:
We have synthesized antifreeze protein III (sequence below), which in
its native state belongs to the beta-sandwhich structural group. Our
synthetic sequence is OK by the usual criteria but we don't seem to be
able to get it to fold properly; i.e., dialysis of a denatured (6 M
GdCl) sample against increasingly dilute GdCl doesn't give a product
with the same CD reported for the native version. Any suggestions,
tricks, etc?

NQASVVANQL IPINTALTLV MMRSEVVTPV GIPAEDIPRL VSMQVNRAVP
LGTTLMPDMV KGYAA

Thanks in advance and best regards,

David Andreu

--
David Andreu
University of Barcelona, Spain
Phone/Fax: 34-934 021 260
andreu@admin.qo.ub.es