We have refolded proteins from freshly deionized urea by diluting
to ca. 0.3 mg/mL in urea, then dialyzing, sometimes stepwise. Low
concentration is important to minimize aggregation.
Chris
>Hi all:
>We have synthesized antifreeze protein III (sequence below), which in
>its native state belongs to the beta-sandwhich structural group. Our
>synthetic sequence is OK by the usual criteria but we don't seem to be
>able to get it to fold properly; i.e., dialysis of a denatured (6 M
>GdCl) sample against increasingly dilute GdCl doesn't give a product
>with the same CD reported for the native version. Any suggestions,
>tricks, etc?
>
> NQASVVANQL IPINTALTLV MMRSEVVTPV GIPAEDIPRL VSMQVNRAVP
>LGTTLMPDMV KGYAA
>
>Thanks in advance and best regards,
>
>David Andreu
>
>--
>David Andreu
>University of Barcelona, Spain
>Phone/Fax: 34-934 021 260
>andreu@admin.qo.ub.es
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427