Peptide Acetylation

Katheryn Resing (Katheryn.Resing@Colorado.EDU)
Thu, 25 Feb 1999 13:08:31 +0000

Hi,
I have a student who wants to acetylate internal lysine residues on
a peptide that represents the first 20 amino acids of histone H4. He has
read about using acetic anhydride in both aqueous and in pyridine/DMF
solvents, but has only the brief description of methods in papers, not the
detailed protocol he would like to have. He has the following questions,
which I am unable to answer. I assured him that there were experts out
there willing to help out, and I append his questions below.

--Katheryn Resing
>
>What is the best way to acetylate a peptide?
>
>What reagents and solvents are the most efficient?
>
>If I am using tritiated acetic anhydride, what is the cleanest and safest
>methods for purification and desalting?
>
>If a detailed protocal could be sent to fax: 303-492-2439 I would be
>very thankful. Any comments on resources can also be sent to
>galasinski@colorado.edu.
>
>Thanks in advance,
>
>Scott Galasinski
>Department of Biochemistry
>University of Colorado, Boulder
>
>galasinski@colorado.edu