Re: HP-1100 diode array HPLC

jim mackintosh (jmackint@proteome.org.au)
Fri, 26 Feb 1999 12:39:09 +1100

Hi, Jim Mackintosh here from APAF, new kid ion the ABRF list actually. We use an
1100 for our N+C sequencer. See this problem on the C-term method which has a
very slow gradient, is annoying as causes flcuations in the RTs. I've always assumed
its a design problem re pumps because I don't see it on N-term methods which ramp
up quickly. I'd also be interested to get any advice on this too.

Cheers, JM

On 25 Feb 99, at 17:36, chris halkides wrote:

> Hello Everyone,
>
> We have an 1100 series hewlett-packard with a diode array detector.
> We sometimes see slow (period of about 5 min) oscillations in the output.
> The pattern is more complex than a simple sine wave. The amplitude is
> about 2 milliabsorbance units both at 220 nm and at 280 nm. It is hard to
> predict when the oscillations will appear or disappear. HP thinks it could
> be the lamp and has suggested turning the flow to zero and seeing what
> happens, but they don't have any further suggestions.
>
>
> Does anyone have any ideas about what is going on, or useful ways
> to proceed?
>
> Chris Halkides
>
> Christopher Halkides
> Dept. of Chemistry, UNCW
> 601 S. College Road
> Wilmington, NC 28403-3297
> (910) 962-7427
>
>

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