Can someone help us please. We are having trouble with our AAA (Pharmacia
Alpha Plus).
We seem to be having a lot of pressure problems. We have thoroughly
cleaned the resin using the recommended protocol, and repacked the column
several times. After this procedure the pressure returns to normal.
When the column is properly packed down, we get an increase in pressure to
90 kg /cm2. It then only takes a few samples for the pressure to get to
danger levels. Yes everything is filtered through a .22 micron filter
including the buffers. We have looked at the resin under a microscope and
there appears to be little damage to the spheres. However there does
apear to be a very small quantity of fibrous material in the resin. (We
must stress only a small amount). Could this be enough to cause this
pressure problem? We have thoroughly cleaned out the lines, and have
established that the problem is resin related by isolating each part of the
system in sequence.
How do we get rid of this material if it is indeed the cause ? Would be
glad of any suggestions as we are desparate. (We can't afford to replace
the resin)
Thanks
Gill Norris and Dave Harding
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David RK Harding
Centre for Separation Science
Institute of Fundamental Sciences
Massey University
Palmerston North
New Zealand
phone 64 6 350-5016 (work) 64 6 356-2837 (home)
fax 64 6 354-2140 (work)
home email Hadaja@xtra.co.nz