HPLC

Deb McMillen (mcmillen@morel.uoregon.edu)
Wed, 03 Mar 1999 11:01:33 -0800

Hi, all
Has anyone ever used a Brownlee CX-300 column? I have one for my old
ABI 120 microbore--and would like to try some cation exchange to
separate a peptide from an 18,000 dalton, pI of 11, protein. What is a
suggested mobile phase? Is 20 mM phosphate pH 5.5, with a gradient up to
0.2 M NaCl a good mobile phase? I want to look at 220nm as the peptide
has no aromatics,
Thanks,
Deb McMillen
Institute of Molecular Biology
University of Oregon
Eugene OR 97403