Re: Cation exchange HPLC
David A. Schooley (schooley@unr.edu)
Wed, 3 Mar 1999 14:49:34 -0800
Deb-
I don't think it will come off under those conditions. I work with
a family of peptides with same pI, and with pH 6.25-6.5 buffer they usually
come off about 0.4-0.8 M NaCl- with of course a different column.
David
David A. Schooley
Dept. of Biochemistry/330
Univ. of Nevada
Reno, NV 89557
schooley@med.unr.edu
tel: (775) 784-4136; fax (775) 784-1419