How do you know it is a 34mer? By sizing on gel or capillary
electrophoresis? If so, you may be observing a hairpin-folded form which
migrates at an apparently smaller size than it really is. Have you checked
the Chimer for self-complementary sequences? Did your instrument trityl
detector suggest the product was OK in manufacture? A mass would help but
I've never seen anything this big on maldi, although there are examples on
the literature on some of the top range mass specs.
Len
>Hi everyone,
>
>I am making a Chimer (RNA & DNA; 5'29DNA+11RNA+6DNA+10RNA+15DNA 3') for a
>user. However, the product is only 34mer. Any suggestions are appriciated.
>
>Best regards,
>
>Bob Lee
*********************************************************************
Dr Len C. Packman
Assistant Director of Research
Protein and Nucleic Acid Chemistry Facility
Department of Biochemistry
University of Cambridge
80 Tennis Court Road
Old Addenbrookes Site
Cambridge, CB2 1GA, UK
Tel: +44 (1223) 333639
FAX: +44 (1223) 766002
e-mail: lcp2@mole.bio.cam.ac.uk
Visit my WWW page at http://www.bio.cam.ac.uk/proj/adr/PNAC/pnac.html