I've never heard about a PVDF membrane causing melts and clogs of the lines.
But, if it is a nitrocellulose membrane, it will be dissolved by ethyl
acetate. And if it is a nylon, it will be dissolved by TFA.
Best regards,
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Prof. Ricardo B. Cunha Brasilia - DF - Brasil
mailto:rbcunha@unb.br
http://www.geocities.com/Athens/Acropolis/5221
Instituto de Quimica - Universidade de Brasilia
http://www.unb.br/ie/qui/
Centro Brasileiro de Servicos e Pesquisas em Proteinas - UnB
http://www.unb.br/cbsp/CBSP.html
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Allison L. Park wrote:
>
> Hello All,
>
> A customer recently submitted a sample to me for protein sequencing on
> Amersham's Hybond-P, which is a PVDF membrane. Before I load it on the
> Procise, I (and my boss) want to know whether it is compatible with the
> sequencing chemistry. Specifically, is this one of those PVDF membranes
> that melts and clogs all of your lines? Does anyone have any experience
> with this membrane?
>
> Thanks,
> Allison Park