DNase and 2D

jaime paba martinez (bucaros@guarany.cpd.unb.br)
Mon, 15 Mar 1999 14:48:19 -0300 (DST)

I have been reading protocols for protein solubilization in 2D, but
they do not talk too much about how to eliminate the contaminant DNA in the
sample. Most of them solubilize the cell extract directly in the
rehydration buffer without any other treatment. So, Is it normally
enough? does the cell extract becomes just a little
viscous? If not, I would appreciate if someone gives me a good reference
or protocol to use DNase and RNase in the procedure.

Thanks in advance,

Jaime Paba
University of Brasilia