Thanks for any info
-- Dr. A. Tarragona Scientific Manager and Honorary Lecturer Advanced Biotechnology Centre BMS division ICSM at CX campus Room 5L.09 St. Dunstan's Road London W6 8RFTel:(0)181-846 1702 Fax:(0)181-846 1055 email: t.tarragona@ic.ac.uk http://abc8.cxwms.ac.uk
---------- >From: dorian immler <dorianimmler@yahoo.com> >To: Recipients of ABRF List <abrf@aecom.yu.edu> >Subject: Re: DNase and 2D >Date: Tue, Mar 16, 1999, 11:30 am >
> > Removing DNA is not necessary in most cases, especially as you work wit= h > small amounts of material as on analytical gels (i.e. 50 - 250 =B5g). > However, an easy way to get rid of DNA is to increase Ampholyt > concentration to >=3D 0.8% (v/v) in the sample buffer and to precipitat= e > DNA by doing so. it will form a translucent pellet after > ultracentrifugation. > > reference: > > rabilloud et al., J. Chromatogr. 1986, 351, 77-89 > > good luck, > > dorian > > Date: > Mon, 15 Mar 1999 14:48:19 -0300 (DST) > From: > jaime paba martinez <bucaros@guarany.cpd.unb.br>Add to Address > Book > Subject: > DNase and 2D > To: > Recipients of ABRF List <abrf@aecom.yu.edu> > > > > > I have been reading protocols for protein solubilization in 2D, but > they do not talk too much about how to eliminate the contaminant DNA in > the > sample. Most of them solubilize the cell extract directly in the > rehydration buffer without any other treatment. So, Is it normally > enough? does the cell extract becomes just a little > viscous? If not, I would appreciate if someone gives me a good > reference > or protocol to use DNase and RNase in the procedure. > > Thanks in advance, > > > Jaime Paba > University of Brasilia > _________________________________________________________ > Do You Yahoo!? > Get your free @yahoo.com address at http://mail.yahoo.com > >=20