we have a single quad from sciex (API100) and have been looking at methods
to determine the mass of the protein backbone (40-80KDa) after
deglycosylation. I wondered what the viewpoint was of using electrospray for
this type of work or would MALDI-MS be more appropriate. Also we have tried
a number of methods to deglycosylate and have found that detergents appear
to be necessary for complete removal of glycans. Any views or opinions on
this would be welcome.
I'm off to the ABRF meeting in North Carolina in a few days and would like
to take the opportunity to speak to anybody who has experience in this area.
If you can offer any advice in this area and would like to chat it would be
much appreciated.
yours
Dougie lamont
PPL Therapeutics
Roslin
Midlothian
EH25 9PP
Tel: 0131-440-4777
Fax: 0131-440-4888
email : Dougie01@globalnet.co.uk (home)
or dlamont@ppl-therapeutics.com (work)