Could you give me reasons why is best to use dome cap tubes compared to flat
cap 0.2 ml tubes.
We have two 9700s and find it pretty difficult to label the tubes. Anachem
is selling flat cap tubes and they say they work for cycle sequencing. Has
anyone tried them.
Your comments will be very much appreciated.
Tony
----------
>From: "Jill Hendrickson" <JillHendrickson@probes.com>
>To: Recipients of ABRF List <abrf@aecom.yu.edu>
>Cc: Recipients of ABRF List <abrf@aecom.yu.edu>
>Subject: Re: Tyrosine fluorescent dye
>Date: Wed, Mar 17, 1999, 11:18 pm
>
> One can label tyrosines with a fluorescent dye in a three-step process:
>
> First, the tyrosine residue in a protein is selectively modified by nitrating
> the ortho position of the phenolic ring using tetranitromethane.
>
> Second, the nitrate is then reduced to an amine using sodium dithionite.
>
> Finally, this new aromatic amine can be reacted _AT LOW pH_ with
> amine-reactive forms of fluorescent dyes, such as isothiocyanates or sulfonyl
> chlorides (succinimidyl esters do NOT work). The lysine and N-terminus amines
> do not react well at low pH, so this reaction will be fairly specific for the
> tyrosine.
>
> We've got plenty of the amine reactive fluorescent dyes at Molecular Probes.
> We have not, unfortunately, got a protocol for this reaction, but here are
> some references in which this reaction has been performed: Biochem Int'l 22,
> 125 (1990) and Biochemistry 18, 3589 (1979).
>
> Here are links on our website that might be helpful:
> http://www.probes.com/handbook/ch03-1.html#Alcohols
> http://www.probes.com/handbook/tables/tab01-1.html
>
> The second link leads to a table. Look under "other" to find which dyes we
> have in isothiocyanate or sulfonyl chloride form.
>
> Hope this helps!
>
> Jill Hendrickson
> Molecular Biology Product Manager
> Molecular Probes, Inc.
>
> "Jack Simpson, Ph.D." wrote:
>
>> --------------
>> Does anyone know if there is such a thing as a fluorescent dye that reacts
>> with tyrosine?
>> -----------------
>>
>> I haven't checked specifically for tyrosine, but Molecular Probes
>> (http://www.probes.com/) has about everything under the sun in terms of
>> fluorescent reagents.
>>
>> Jack
>>
>> ~~~~~~~~~~~~~~~~~~
>> Jack Simpson, Ph.D.
>> Pharmakinetics Laboratories
>> 302 West Fayette Street
>> Baltimore, MD 21201
>> 410-385-4500, ext. 688
>> jsimpson@pharmakinetics.com
>> http://sx102a.niddk.nih.gov/mass/lnt/simpson.html
>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
>
>