Re: Edman Degradation/Asp

John Hempel (hempel@psc.edu)
Tue, 30 Mar 1999 14:35:00 -0500

Paul,
It's mentioned (altho not in any detail) in Allen, Sequencing of Proteins
and Peptides (=Laboratory techniques in Biochemistry & Molecular Biol vol
9 (Elsevier, 1989), p80; see also p232. Another likely source would be
Bornstein & Balian, Meth Enzymol 47:132, who list 40+ refs in their
discussion of cleavage with hydroxylamine.

Regards,
John
At 10:42 AM 3/30/99 -0500, you wrote:
>I've been sequencing a peptide with several Asp residues, one of which
>readily forms an aspartimide and which can be readily induced to form a
>beta-Asp. Sequencing by Edman degradation does not proceed beyond the
>beta-Asp and proceedes slowly through the aspartimide (good diagnostic
>tools in conjunction with MS, chemical reversion, and Asp-N peptide
>mapping). I know chemically why this should happen and can draw clear
>diagrams, but I haven't been able to find a literature reference to the
>Edman chemistry as it relates to the aspartimide. Has anyone run across
>one that might be helpful? Are there any other amino acid modifications
>that affect Edman degradation rates, but not the final PTH derivative?
>
>Thanks for your help.
>Paul Tressel
>
>
John Hempel, PhD Ph (412) 624 0161
University of Pittsburgh FAX (412) 624 4759
Department of Biological Sciences
Pittsburgh PA 15260 email: hempel@psc.edu
http://www.pitt.edu/~biology/faculty/hempel.html