See Methods Enzymology 47, 132, 1977.
Se also some Edmans thru one in Takio et al, Biochemistry 25, 8049, 1986.
Kenneth A. Walsh
E.W.Davie/ZymoGenetics Chair of Biochemistry
Box # 357350
University of Washington
Seattle WA 98195
walsh@u.washington.edu
Phone 206-543-1768
FAX 206-685-9231
On Tue, 30 Mar 1999, Paul Tressel wrote:
> I've been sequencing a peptide with several Asp residues, one of which
> readily forms an aspartimide and which can be readily induced to form a
> beta-Asp. Sequencing by Edman degradation does not proceed beyond the
> beta-Asp and proceedes slowly through the aspartimide (good diagnostic
> tools in conjunction with MS, chemical reversion, and Asp-N peptide
> mapping). I know chemically why this should happen and can draw clear
> diagrams, but I haven't been able to find a literature reference to the
> Edman chemistry as it relates to the aspartimide. Has anyone run across
> one that might be helpful? Are there any other amino acid modifications
> that affect Edman degradation rates, but not the final PTH derivative?
>
> Thanks for your help.
> Paul Tressel
>
>