Re: receptor-ligand model systems
chris halkides (halkidesc@UNCWIL.EDU)
Thu, 01 Apr 1999 18:36:41 -0400
>Date: Thu, 1 Apr 1999 17:48:37 -0400
>To: David J Detlefsen
>From: chris halkides <halkidesc@uncwil.edu>
>Subject: Re: receptor-ligand model systems
>Cc:
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>Hello Dave,
>
> Are reversible covalent interactions OK? There are a number of
>peptidyl aldehdye inhibitors of proteases which are commercially
>available, such as calpain and leupeptin.
>
>Chris Halkides
>
>
>
>
>
>>Hello,
>>
>>I'd like to toss out a question to the group. Can anyone recommend a
>>model system for receptor-ligand binding?
>>
>>Here are the criteria for the ideal system:
>>1. commercially available
>>2. ligand = small molecule (like a drug) & receptor = protein
>>3. complexation is reversible & no covalent attachment
>>4. complex affinity in the mM to sub uM range
>>5. a range of ligands with different affinities
>>
>>Obvious things that come to mind are proteases but there are precious
>>few (at least that I can find) protease inhibitors that meet the above
>>criteria. Also, I'm looking at lysozyme interacting with tri-NAG. This
>>seems like a great system but I can't find a source for tri-NAG (and I
>>don't know if there are similar ligands with lesser affinity).
>>
>>Any help/comments/suggestions would be appreciated.
>>
>>Thanks in advance.
>>
>>Dave Detlefsen
>>Bristol-Myers Squibb
>
Christopher Halkides
Dept. of Chemistry, UNCW
601 S. College Road
Wilmington, NC 28403-3297
(910) 962-7427