This said, I had two observation:
1) I use a flow synthesized, not a batch, the risk with a batch is that the
fmoc leaving the resin, that normally react with piperidine, do not react with
DBU, and you may have side reaction with your peptide (that the reason of the
1% Piperidine in my system).
2) Do not, do not use it with ASP(I use Fmoc-D(otBu) , it will lead to a
dehydration with lost of 18 Da from your peptide.
I had a poster at the 15 APS with some comparison between DBU and Piperidine.
M. Villain, P. L. Jackson, N.R. Krishna and J.E. Blalock,1999. Application of
different coupling and deprotection conditions in the synthesis of "difficult
sequences". In : Peptide: Frontiers of Peptide Science (J.P. Tam and P.T.P.
Kaumaya, Eds.). Proceeding of the 15th American Peptide Symposium,, June
14-19, 1997, Nashville, TN,. Kluwer Academic Publishers, Dordrecht. Pp. 255-256
N.B. it has been finally published, thank you Professor TAM!!!
Terry Stoming wrote:
> Does anyone have recent information on the use of DBU in peptide synthesis?
-- Matteo Villain E-mail : villain@uab.edu Research Associate Phone : 205 934 3032 University of Alabama Fax : 205 934 1446 Birmingham 35294, USA