Missing tryptophan in N-terminal Sequencing

Suzanne Perry-Riehm (perry@interchange.ubc.ca)
Wed, 7 Apr 1999 13:57:30 -0700 (PDT)

I have a peptide sequence in which the tryptophan is "missing" in the
cycle where it should have appeared. The original sample was a protein
digested
after R&A with endo LYS-C. The peptides were separated on a PE ABI173A
and the peptide peak sequenced with standard gas-phase Edman Chemistry. I
do visualize a small peak that co-elutes with PRO. The client assures me
that this does have a TRP in this position and the rest of the sequence
matches up 100%.

Any help in this matter?

Thanks,
Suzanne Perry-Riehm

Suzanne C. Perry, Research Associate
NAPS Protein Service Laboratory
Biotechnology Department, University of British Columbia
CANADA
phone:(604)822-9662 Fax:(604)822-0676 email:perry@interchange.ubc.ca
Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm