I have a different sort of question and I thought this to be as good a
place as any to start.
We are doing metal chelation work of peptides/proteins with analysis by
ICP-MS. Our problem is that using DTPA/EDTA etc. (aqueous soluble
chelators) that we cannot remove the chelator-metal complex from the buffer
(usually PBS) prior to analysis. Thus we have been using 'generic' resins
that remove all metals/cations and so forth.
Does anybody know of specific metal resin bound chelators or alternatively,
know of a method of removing chelor-metal complexes from solution? I have
heard in the petrochemical industry they do some aqueous-organic extraction
but have no idea where to start looking or indeed what on earth to look for.
Thanks in advance
Regards to all
Michael Jobling
m.jobling@pgrad.unimelb.edu.au