The presence of this small peak near Pro is a secondary product of
PTH-Trp. In our Procise, using either gas or pulse liquid chemistry, we
see this peak and always it is associated with the presence of TRP.
I hope this info could be useful.
Jose Cesar Rosa, Ph.D
Centro de Quimica de Proteinas
Faculdade de Medicina Ribeirao Preto
Universidade de Sao Paulo
Av. Bandeirantes, 3900
14049-900 Ribeirao Preto, Brazil
phone: 55-16-6331884
fax: 55-16-6332119
email: jcrosa@fmrp.usp.br
On Wed, 7 Apr 1999, Suzanne Perry-Riehm wrote:
> I have a peptide sequence in which the tryptophan is "missing" in the
> cycle where it should have appeared. The original sample was a protein
> digested
> after R&A with endo LYS-C. The peptides were separated on a PE ABI173A
> and the peptide peak sequenced with standard gas-phase Edman Chemistry. I
> do visualize a small peak that co-elutes with PRO. The client assures me
> that this does have a TRP in this position and the rest of the sequence
> matches up 100%.
>
> Any help in this matter?
>
> Thanks,
> Suzanne Perry-Riehm
>
> Suzanne C. Perry, Research Associate
> NAPS Protein Service Laboratory
> Biotechnology Department, University of British Columbia
> CANADA
> phone:(604)822-9662 Fax:(604)822-0676 email:perry@interchange.ubc.ca
> Visit our web page at : www.biotech.ubc.ca/naps/napshome.htm