______________________________ Reply Separator _________________________________
Subject: Re: antibody analysis
Author: Dan Crimmins <crimmins@pathbox.wustl.edu> at SMTP
Date: 4/9/99 7:58 AM
At 01:26 PM 4/8/99 -0700, Michael Moore wrote:
>Does anyone have a suggestion for measuring the amount of agragated vs.
>non-agragated monoclonal antibody. The MW is approximately 150kD, and the
>agragates are most likely greater than dimers. Thanks in advance
>Michael
>
>
>
>
Michael,
If you only need to know 150kD species vs. everything else, size
exclusion
chromatography will do the job. A G4000SW works well for this. It is
usually not possible to assign meaningful MW's to the higher MW species
though.
Regards,
Dan L. Crimmins
Washington University School of Medicine
Dept. Pathology/Division of Laboratory Medicine
660 S. Euclid Ave., Box 8118
St. Louis, MO 63110
Phone: 314-454-8514; Fax: 314-454-5208
e-mail: crimmins@labmed.wustl.edu